3-hydroxy-3-methylglutaryl-CoA reductase (HMGR) is the rate-limiting enzyme of mevalonate pathway, which is the metabolic pathway of acetyl-CoA to produce cholesterol.This detection kit can screen inhibitors of HMGR, which is an important tool for basic research of cholesterol and other related metabolic pathways.<br/>
The detection principle of this kit: HMGR catalyzed substrate reaction consumes NADPH, and the absorbance decreases at 340 nm. The addition of inhibitors will inhibit HMGR enzyme activity, resulting in a decrease in the rate of absorbance decline. The inhibition rate can be calculated by measuring the change value of absorbance.
