Fructose-6-phosphate (F-6-P) is a six-carbon sugar formed by esterification of fructose and phosphate. It is an important intermediate in glycolysis and gluconeogenesis pathways. As the most basic metabolic substances in the metabolic process of living bodies and cells, it is of great significance to study them quantitatively.
Enzyme catalyze F-6-P to produce a specific product, which can react with chromogenic agent to produce the fluorescent substance. Detect the fluorescent intensity of fluorescent substance at the excitation wavelength of 535 nm and the emission wavelength of 587 nm.
