NEFA and can react with coenzyme A and form acetyl-CoA under the catalysis of acetyl-CoA-synthetase (ACS). Acetyl-CoA can produce H2O2 when catalyzed by acetyl-CoA-oxidase (ACOD). Then H2O2 react with TOOS and 4-amino-antipyrine (4-APP) to generate a colored substrate under the catalysis of peroxidase (POD). The colored substrate has a maximum absorption peak at 546 nm. Measure the OD value at 546 nm and calculate the NEFA content indirectly.

Codice: E-BC-K014_96T | Marca: Elabscience | Confezionamento: 96T

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Codice: E-BC-K014_96T
Marca: Elabscience
Link: Apri link
Stoccaggio: This product can be stored at 2~8°C for 12 months with shading light.
Detection range: 0.01-3.0 mmol/L
Metodo di rilevazione: Microplate reader;Biochemistry analyzer
Tipo di campione: serum;plasma;tissue homogenate;cells;cell supernatant
Ig target: NEFA
Area di ricerca: Metabolic Diseases;Glycolysis and Lipid Metabolism

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