Myeloperoxidase reduces hydrogen peroxide to a complex. The complex react with o-dianisidine (as hydrogen donor) to produce a yellow product which has a maximum absorption peak at 460 nm. The activity of MPO can be calculated indirectly by measuring the OD value at 460nm.
Under the catalysis of peroxidase, hydrogen peroxide can oxidize the non-fluorescent probe into the fluorescent substance, and its fluorescence intensity is proportional to the total peroxidase activity in the sample. This kit specifically inhibits the peroxidase activity of MPO in the sample through an MPO enzyme inhibitor, thus distinguishing the peroxidase activity of MPO in the sample from that of other peroxidases.
NADH Oxidase（NOX）is widely found in the animals, plants, microorganisms and cultured cells, which can directly oxidize NADH to NAD+ in the presence of oxygen, and reduce blue DCPIP to colorless DCPIP. The activity of NOX can be calculated by measuring the reduction rate of blue DCPIP at 600 nm.
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