Neuraminidase (NA), also known as sialase, is a family of glycolipid, oligosaccharide and glycoprotein terminal sialic acid residues that can be hydrolyzed, distributed in viruses, bacteria, fungi and fungi. In vertebrate cells. The detection principle of the test box is to calculate the neuraminidase activity of the sample by detecting the production rate of fluorescent substances per unit time.
Neuraminidases, also known as sialidase, are a family of exoglycans that hydrolyse glycolipids, oligosaccharides and sialic acid residues at the end of glycoproteins. NA is distributed in viruses, bacteria, fungi and vertebrate cells. Viral neuraminidase is one of the important targets of some influenza drugs. Human neuraminidase plays a role in a variety of signaling pathways and is associated with a variety of diseases such as neurodegenerative diseases, cancer, and cardiovascular and cerebrovascular diseases. Therefore, it is important to develop NA inhibitors. The detection principle of this kit is that NA decomsolves the substrate to release fluorescent substances, and the activity can be inhibited by adding NA inhibitor. The inhibition ability of inhibitor can be judged by the fluorescence value.
Neutrophil Elastase (NE) is one of the main serine proteases secreted by neutrophils. It plays an important role in the physiological and pathological processes of the body, on the one hand NE participate in the formation of the body's defense system and assist in the elimination of pathogenic microorganisms. On the other hand, the excessive activation of NE, in the case of imbalance with endogenous inhibitors, is directly involved in tissue damage, and is closely related to the occurrence of a variety of inflammation-related diseases, such as atherosclerosis, chronic obstructive pulmonary disease, pulmonary cystic fibrosis and other diseases, which can be used as a prevention and treatment target for these diseases. The detection principle of this kit is as follows: NE hydrolyzates the substrate to generate chromogenicity substances, which have maximum absorption at the wavelength of 380 nm, and NE activity in the sample is calculated by measuring the OD value at 380 nm and the standard curve.
Nitric oxide (NO) is chemically active and is rapidly metabolized in the body to nitrite (NO2-) and nitrate (NO3-), and NO2- is further converted into NO3-. In this kit, NO3- was reduced to NO2- by reductant agent, and reacts with chromogenic agent to produce color substance. The nitrate content can be determined at 540 nm.
NO is easily oxidized to form NO2- in vivo or in aqueous solution, and a reddish azo compound is formed with the color developing agent, and the concentration of the azo compound is linearly related to the concentration of NO. The concentration of NO can be calculated indirectly by measuring the OD value at 550 nm.
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