Oxalic, also known as ethanedioic acid, is a simple organic acid in plants and plays important physiological roles. Oxalic participates in the regulation of Ca2+, pH and osmotic pressure within cells, as well as the defense responses of plant pathogens and the alleviation of aluminum toxicity. Although Oxalic has important physiological functions, excessive intake of Oxalic can also cause many adverse effects on the human body. Oxalic affects the absorption of mineral elements such as calcium and iron by the human body, increases the risk of developing kidney stones, inflammation, hyperoxaluria, uremia and other diseases, and in severe cases, it can even cause acute Oxalic poisoning. Moreover, oxalic is not easily oxidized and decomposed in the human body, and its metabolites can disrupt the acid-base balance in the body. The detection principle of this kit: Oxalate oxidase catalyzes the oxidation of oxalate to produce hydrogen peroxide and carbon dioxide. Hydrogen peroxide reacts with chromogenic substances under the action of enzymes to form colored products. There is a maximum absorption peak at 550 nm, and the color depth is proportional to the content of oxalic.
Oxaloacetic acid (OAA), one of the intermediates in the tricarboxylic acid cycle is an important substance in carbon and nitrogen metabolism. Oxaloacetate reacts with substrate acetyl-coA under the action of enzyme. The substance produced can react with DTNB, which has the maximum absorption peak at 412 nm. The content of oxaloacetate can be determined by measuring the absorbance value.
Mitochondrial oxidative phosphorylation consumes oxygen to produce ATP, which provides energy for cell growth. Therefore, detection of cellular oxygen consumption is a key indicator of mitochondrial function. The kit provides a fluorescent probe, which is sensitive to oxygen. The fluorescence of the probe increases with the decrease of oxygen in a closed environment, and the oxygen consumption rate of cells is judged by detecting the change of the fluorescence value.
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