Plant peroxidase, a member of the superfamily of peroxidase, catalyzes the redox reaction between H2O2 and various reductants. The plant peroxidase has the same general structure and consists of iron porphyrin IX and ten α-helixes. Based on the difference of primary structure, the superfamily of plant peroxidase can be divided into three types: class I (intracellular type), class II (extracellular type of fungi) and class III (secreted type of plant).
The peroxidase can catalyze the decomposition of H2O2 and produce water and oxygen. And oxygen oxidized pyrogallic acid to form yellow product. The activity of peroxidase can be calculated by measuring the absorbance at 420 nm.
Phenylalanine ammonia lyase (PAL) can catalyze L-phenylalanine to produce trans-cinnamic acid and ammonia, and trans-cinnamic acid has the maximum absorption peak at 290 nm. PAL activity can be calculated by measuring the increase of OD value at 290 nm.
Phenylalanine (Phe), chemically known as 2-amino-3-phenylpropionic acid, is an essential amino acid involved in a variety of biosynthetic processes. In the body, most Phe is oxidized to tyrosine by phenylalanine hydroxylase, and together with tyrosine, it synthefies important neurotransmitters and hormones, and participates in glucose metabolism and fat metabolism. Phenylalanine is catalyzed by enzyme , and the chromogenic agent has an absorption peak at 450 nm. The Phe content of the sample can be calculated by measuring the OD value of the chromogenic substance at 450 nm.
The principle is that the compounds formed by phosphoric acid and molybdate under acidic conditions will form color substances with malachite green. The depth of color is proportional to the content of phosphate. The phosphorus content can be calculated indirectly be measuring the OD value at 660 nm.
Phosphatidylcholine (PC) is an important component of eukaryotic cell membranes and a major source of second messenger diacylglycerol, phosphatidic acid, lysophosphatidic acid, and arachidonic acid, which can be further metabolized into other signaling molecules. It is widely found in a variety of foods, especially egg yolks, soy, fish, meat, and some seeds, and it can also be consumed through dietary supplements. PC provides structural stability and fluidity of cell membranes. In the liver, PC is involved in lipid synthesis and transport, helping to maintain lipid balance. As an important component of bile, PC promotes the digestion and absorption of lipid. PC supplements are used to intervene and treat liver diseases such as fatty liver disease and cirrhosis, and are used by athletes to improve physical fitness and endurance. Therefore, the detection of PC has important application value in medical diagnosis, disease management and health evaluation. The detection principle of this kit: Enzyme catalyze the PC and chromogenic agent to produce, with maximum absorption at 550 nm, and the content of PC in the sample can be calculated by measuring the OD value at 550 nm.
Lascia la tua email e seleziona le aree che vuoi seguire per ricevere novita di prodotto, approfondimenti tecnici e promozioni piu pertinenti.
Puoi scegliere le macro-aree Life Science e Diagnostica oppure le singole specializzazioni.
Seleziona uno o piu ambiti di interesse per ricevere comunicazioni piu utili e mirate.
Aggiornamenti su ricerca, strumentazione e applicazioni per il mondo Life Science.
Novita, approfondimenti e promozioni per laboratori diagnostici e flussi operativi.
2025 TEMA Ricerca - All Rights Reserved.
