Phosphoenolpyruvate carboxylase (PEPC) catalyzes phosphoenolpyruvate (PEP) to produce oxaloacetic acid, which further reacts with NADH. NADH has maximum absorption at 340 nm. The activity of PEPC can be calculated by measuring the change of absorbance value at 340 nm.
Phosphofructokinase(also known as 6-phosphofructokinase; PFK) is a class of kinases that act on fructose-6-phosphate. Phosphofructokinase catalyzes fructose-6-phosphate and ATP to produce fructose-1, 6-diphosphate and ADP, and pyruvate kinase and lactate dehydrogenase further catalyze the oxidation of NADH to NAD+. The activity of PFK can be reflected by the determination of NADH decline rate at 340 nm.
Phosphoglucose Isomerase (PGI) is an important enzyme that can catalyze the interconversion between glucose-6-phosphate (G6P) and fructose-6-phosphate (F6P), and is involved in glycolysis and the pentose phosphate pathway. These processes help cells break down glucose for energy and generate key substances in other metabolic pathways. The detection principle of this kit is as follows: The PGI catalyze the substrate to produce the product, reacting with chromogenic agent to form the chromogenic substance, which has the maximum absorption at a wavelength of 450 nm. The PGI enzyme activity is calculated by measuring the OD value at 450 nm.
Phospholipase C (PLC) is a hydrolase that breaks down phosphatidylinositol diphosphate (PIP2) to form diacylglycerol (DAG) and inositol triphosphate (IP3). PLCS are widely found in bacteria, plants and animals. Its abnormal expression is associated with a variety of cancers, and studying the PLC mechanism can help develop new anticancer therapies. PLC also play a key role in nerve signaling and protection, helping to understand and treat neurodegenerative diseases. In addition, PLC plays an important role in insulin signaling and lipid metabolism, making it a hot topic in diabetes and obesity research. PLC catalyzes the substrate to produce chromogenic substance has maximum absorption at 410 nm, and the PLC enzyme activity is calculated by measuring OD value at 410 nm.
Phospholipase D (PLD) exists widely in natural plants and animals, and PLD plays a key role in phospholipase metabolism. PLD hydrolyzes phosphodiester bonds of glycerol phospholipids to produce phosphatidylic acid and nitrogenous base. Abnormal PLD activity and expression are associated with Alzheimer's disease, stroke, cancer, and other brain diseases. The detection principle of this kit is that PLD catalyze the substrate and the chromogenic agent to product colored substance, which has the maximum absorption at the wavelength of 550 nm. By measuring the OD value at 550 nm, PLD activity in the sample is calculated according to the standard curve.
Inorganic phosphorus react with molybdic acid to produce phosphomolybdic acid. Phosphomolybdic acid can be reduced to molybdenum blue under the action of reducing agent. And the molybdenum blue have a maximum absorption peak at 660 nm. The phosphorus content can be calculated indirectly be measuring the OD value at 660 nm.
Lascia la tua email e seleziona le aree che vuoi seguire per ricevere novita di prodotto, approfondimenti tecnici e promozioni piu pertinenti.
Puoi scegliere le macro-aree Life Science e Diagnostica oppure le singole specializzazioni.
Seleziona uno o piu ambiti di interesse per ricevere comunicazioni piu utili e mirate.
Aggiornamenti su ricerca, strumentazione e applicazioni per il mondo Life Science.
Novita, approfondimenti e promozioni per laboratori diagnostici e flussi operativi.
2025 TEMA Ricerca - All Rights Reserved.
