Inorganic phosphorus react with molybdic acid to produce phosphomolybdic acid. Phosphomolybdic acid can be reduced to molybdenum blue under the action of reducing agent. And the molybdenum blue have a maximum absorption peak at 660 nm. The phosphorus content can be calculated indirectly be measuring the OD value at 660 nm.
Phytase also known as inositol hexaphosphatase, is a protein and sugar binding enzyme. Phytase can catabolize phytic acid to produce inorganic phosphorus and inositol, which greatly improves nutrient utilization rate of organisms. Natural phytase is widely found in plants, animal tissues and microorganisms. Phytase has a wide research value in the field of food production and animal husbandry. Under certain environmental conditions, phytase can decompose sodium phytate to produce inorganic phosphorus and inositol derivatives. Under acidic conditions, inorganic phosphorus and ammonium molybdate chromogenic agent react to produce a blue molybdenum blue substance, which has a characteristic absorption peak at 700 nm. Phytase activity can be calculated by measuring the content of inorganic phosphorus.
Ammonium nitrogen reacts with hypochlorite and phenolic substances in a strong alkaline medium to produce water-soluble dye indigophenol blue, which has a characteristic absorption peak at 625nm, and the absorption value is proportional to the content of ammonium nitrogen.
In alkaline nitrite solution, flavonoids form red complex with aluminum ion. The flavonoid content of the sample can be calculated by measuring the absorptivity of the sample extract at 510 nm.
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