In alkaline nitrite solution, flavonoids form red complex with aluminum ion. The flavonoid content of the sample can be calculated by measuring the absorbance of the sample extract at 510 nm.
In alkaline nitrite solution, flavonoids form red complex with aluminum ion. The flavonoid content of the sample can be calculated by measuring the absorptivity of the sample extract at 510 nm.
In the first step, protoplasts were prepared by enzyme. In the second step, mitochondrial organelles were released by membrane breaking solution. In the third step, purified mitochondria were obtained by differential centrifugation.
Nitrate nitrogen can react with salicylic acid to produce nitrosalicylic acid in a strongly acidic environment. Nitrosalicylic acid is yellow under strong alkaline conditions, and the depth of color is proportional to the content at a wavelength of 410 nm.
The detection principle of the kit is to use 2，3，5-triphenyltetrazolium chloride (TTC) as the substrate, incubate for 1 to 3 hours, the dehydrogenase in the root can reduce TTC and generate 1,3,5-tiphenylformazan (TTF) which is insoluble in water, then TTF will be extracted from the root with organic solvent (ethyl acetate or acetone, etc.) By detecting the absorbance at 485nm, the amount of TTC reduction can be calculated, which represents the dehydrogenase activity and is used as an index of plant root activity. The kit is used for 含量 determination of plant root activity or dehydrogenase activity.
Carbohydrate is one of the important components of plant corpus, and is also the main raw material of metabolism and storage material. The kit is used for the determination of soluble monosaccharides, oligosaccharides and polysaccharides. It has the advantages of high sensitivity, quick convenience and suitable for the determination of microscale samples. The detection principle is anthrone colorimetry. Carbohydrate react with anthrone to produce colored material, which has the maximum absorption peak at 620 nm. The soluble sugar content can be determined by measuring the absorbance value.
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