Polyphenol oxidase (PPO) can catalyze phenolic compounds into quinone substances. The latter has specific absorption at 410 nm. The activity of PPO can be calculated indirectly bymeasuring the OD value at 410 nm.
Polyphenol oxidase (PPO) can catalyze phenolic compounds into quinone substances. The latter has specific absorption at 410 nm. The activity of PPO can be calculated indirectly by measuring the OD value at 410 nm.
Under the alkaline condition, the sodium tetraphenylborate reacts with the potassium ions in the sample to form the potassium tetraphenylborate which is white and small particles with small solubility. Potassium tetraphenylborate particles are in a stable suspension state in the solution. The turbidity is proportional to the potassium ion concentration in the sample and potassium content can be calculated indirectly by measuring the OD value at 450 nm.
The hydrogen produced by proline during the catalytic process of ProDH can reduce the chromogenic agent through electron acceptor, and lighten the color. The ProDH activity can be characterized by measuring the rate of absorbance decrease at 600 nm.
Proline can react with acidic-ninhydrin to form stable red compound. The maximum absorption peak of the compound is at 520 nm. And the concentration of Pro can be calculated by measuring the OD value at 520 nm
Proline can react with acidic-ninhydrin to form stable red compound. The maximum absorption peak of the compound is at 520 nm. And the concentration of Pro can be calculated by measuring the OD value at 520 nm.
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