Proline can react with acidic-ninhydrin to form stable red compound. The maximum absorption peak of the compound is at 520 nm. And the concentration of Pro can be calculated by measuring the OD value at 520 nm
The content of protein carbonyl increased after oxidation, and the carbonyl group reacted with 2, 4-dinitrophenylhydrazine to form a reddish brown precipitate. The absorbance can be measured at 370 nm after the precipitation is dissolved. The carbonyl content can be calculated indirectly.
Protein Tyrosine Phosphatase-1B (PTP-1B) belongs to the family of protein tyrosine phosphatases, which specifically hydrolyze aromatic phosphates and negatively regulate insulin signal transduction by dephosphorylating tyrosine residues on insulin receptors or their substrates. In the dephosphorylation of PTP-1B, the phosphate group produced reacts with malachite green to form chromogenic substance, PTP-1B in samples is measured by measuring the absorbance change at 636 nm.
The product of the PC-catalyzed substrate reacts with the reducing agent. The reducing agent has a maximum absorbance at 340 nm, and the enzyme activity of PC is calculated by calculating the amount of reducing agent consumed in the reaction.
Pyruvate decarboxylase (PDC) mainly exists in yeast and is one of the key enzymes in ethanol fermentation. PDC catalyzes pyruvate decarboxylate to acetaldehyde, which reacts under the action of ethanol dehydrogenase (ADH), and catalyzes NADH to convert into NAD+. NADH has a characteristic absorption peak at 340 nm. The PDC activity can be calculated by measuring the OD value at 340 nm.
Pyruvate dehydrogenase (PDH) catalyzed the oxidative decarylation of pyruvate into acetyl CoA, which plays an important role in the metabolism of mitochondrial respiratory chain and is associated with the aging of cancer genes. Pyruvate can be decarboxylated by PDH under the action of CoA. Meanwhile, NAD+ is reduced to NADH, Which under the action of hydrogen transmitter, transfer electrons to WST-8 to produce the yellow product. The activity of PDH can be calculated by measuring the change of absorbance value at 450 nm.
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