TBARS and TBA can react under high temperature and acid conditions and then form a pink compound, the concentration of which is linearly related to the concentration of TBARS in the sample. The TBARS concentration can be calculated by measuring the fluorescence values at the excitation wavelength of 530 nm and the emission wavelength of 550 nm.
Thioredoxin system, as one of the most important antioxidant systems in the body, can stabilize the redox balance of organisms, regulate signal transduction. It is also related to cell REDOX reaction, nucleic acid metabolism, cell growth and tumorigenesis. Thioredoxin reductase (TrxR) is a NADPH-dependent dimer selenase containing the flavin adenine dinucleotide (FAD) domain and is a member of the pyridine nucleotide-disulfide oxidoreductase family. The thioredoxin system consists of NADPH, thioredoxin reductase and thioredoxin. The TrxR catalytic substrate makes the reducing agent reduce the color developer with a characteristic absorption peak at 412 nm.
Copper ions can complex with various amino acids to produce blue-green complex compound, and the depth of color is proportional to the content of total amino acids at a specific wavelength. T-AA content can be calculated with the absorbance at 650 nm.
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