Triglyceride is converted by enzyme to produce hydrolytic products, which is catalyzed by enzymes to produce fluorescent substances. The content of triglyceride in samples can be calculate by measuring the fluorescence value.
The color depth of the generated quinones is directly proportional to the triglyceride content. The absorbance values of the standard tube and the sample tube are measured respectively, and the triglyceride content in the sample can be calculated.
Triglycerides (TG) can be hydrolyzed by lipoprotein lipase into glycerol and free fatty acids. Glycerol produces glycerol-3-phosphate and ADP under the catalysis of glycerol kinase (GK). Glycerol-3-phosphate produces hydrogen peroxide under the action of glycerol phosphate oxidase (GPO). In the presence of 4-aminoantipyrine and phenol, hydrogen peroxide is catalyzed by peroxidase (POD) to produce quinones which is proportional to the content of TG.
Trypsin catalyzes the substrate to form p-nitroaniline, which has a certain light absorption value at 400-410 nm wavelength. Since the absorbance of p-NA is proportional to the content, the activity of trypsin can be calculated by measuring the p-NA produced per unit time
Tryptophan (Trp) is an essential amino acid involved in a variety of biosynthetic processes. It is not only involved in the synthesis of a variety of proteins, but also the precursor of many bioactive substances. The enzyme catalyzes Trp in a series of reactions to produce hydrogen peroxide, which forms a red complex with the fluorescent probe. The fluorescence value of the sample at the excitation wavelength of 535 nm and the emission wavelength of 587 nm can be measured to calculate the content of Trp in the sample.
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