The protein in urine reacts with Coomassie Blue G-250 under acidic conditions to produce chromogenic substance. The reaction system changes from brownish red to blue. The blue substance produced has the maximum absorption wavelength at 585-605 nm.
The most obvious chemical activity of VC is that reduce Fe3+ to Fe2+, then promote iron absorption in the intestine, promote the storage and utilization of iron. Fe3+ react immediately with reducing ascorbic acid to form Fe2+. then Fe2+ react with phenanthroline and the color developing reaction occurs. The content of vitamin C in sample can be determined. Measure the OD value and calculate the VC content indirectly.
Fe3+ can be deoxidized to Fe2+ by VE with ferroin existing. Fe2+ can react with phenanthroline and form pink compound under certain condition. VE content can be calculated by measuring the OD value at 532 nm.
Fe3+ can be deoxidized to Fe2+ by VE with ferroin existing. Fe2+ can react with phenanthroline and form pink compound under certain condition. After colorimetric assay, VE content can be figured out according to the standard curve or calculated through formula.
Xanthine and hypoxanthine are important alkaloid purines that can be used to treat symptoms such as asthma relief and bronchodilation. Hypoxanthine can be oxidized by xanthine oxidase to form xanthine. Xanthine is catalyzed by xanthine oxidase to form uric acid. When xanthine oxidase is deficient, xanthine accumulates in urine and blood, inducing hyperuricemia and gout, and eventually leading to renal failure. The detection principle of this kit: Xanthine/hypoxanthine generates intermediate products under the catalysis of enzymes. Under the action of other enzymes, the intermediate products react with the probe to form fluorescent substances. By substituting the fluorescence values into the standard curve, the total content of xanthine and hypoxanthine in the sample can be calculated.
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