Phenol and sodium hypochlorite react with GABA to produce a blue-green product, which has maximum absorbance at 640 nm. GABA content can be calculated with the absorbance at 640 nm.
γ-Aminobutyric Acid (GABA) is a major inhibitory neurotransmitter in the mammalian central nervous system. GABA also has the function of promoting mental stability, regulating blood pressure level, improving brain vitality, increasing growth hormone secretion, accelerating ethanol metabolism and providing nutrients to nerve cells. GABA is catalyzed by enzyme, and the removed hydrogen will reduce NADP+ to NADPH, and the GABA content of the sample can be calculated by measuring the generated amount of NADPH.
γ-GT catalyzes the transfer of gamma glutamyl group from glutamyl p-nitroaniline to N-glycyl glycine to produce p-nitroaniline, which has characteristic absorption peak at 405nm. The activity of γ-GT can be calculated according to the changing rate of absorbance value.
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