Using NAD+ as H+ receptor, D-lactate dehydrogenase (LDH) catalyzes the reaction of D-lactic acid and NAD+ to generate pyruvic acid and NADH respectively. NBT is reduced to a kind of purple compound during the reaction. Measure the OD value at 530 nm, and the concentration of D-lactic acid can be calculated.#N/A
DPPH is a synthetic organic free radical that can be used to evaluate the antioxidant activity of antioxidants. Its single electron has the maximum absorption at 525 nm, and the DPPH radical scavenging ability of the sample can be calculated by measuring the change of OD value before and after the addition of antioxidants.
Dual luciferase reporter gene uses luciferin to detect the luminescence reaction produced by luciferin substrate. The detection system includes two parts: firefly luciferase reporter gene and renilla luciferase reporter gene. As an internal parameter of transfection, the renilla luciferase can normalize the experimental data. The reaction principle of this test box is that in the presence of oxygen, ATP and magnesium ions at the same time, firefly luciferase and coelentin are catalyzed and oxidized successively by firefly luciferase and renilla luciferase in the samples to produce different fluorescence signals, and the expression levels of firefly luciferase and renilla luciferase in the samples can be detected by chemiluminescence instrument.
Dual luciferase reporter gene is used to detect the luminescence reaction produced by luciferase catalyzed substrates. The detection system includes firefly luciferase reporter gene and renilla luciferase reporter gene. As an internal parameter of transfection, the renilla luciferase can normalize the experimental data. Firefly luciferase reporter gene refers to a reporting system that uses fluorescein as substrate to detect fluorescein luciferase activity. The reaction can occur in the presence of ATP, magnesium ions and oxygen, resulting in yellow-green fluorescence signals. The advantage of the reporter gene is that the expression product of the reporter gene has detectable enzyme activity as soon as the translation is completed. Renilla luciferase reporter gene refers to a reporting system that uses coelenterazine as substrate to detect the activity of renilla luciferase. This reaction does not depend on ATP to participate in the enzymatic reaction. During the reaction, a blue fluorescence signal is generated. The reaction principle of this kit is that in the presence of oxygen, ATP and magnesium ions at the same time, firefly luciferin and coelenterazine are catalyzed and oxidized successively by firefly luciferase and renilla luciferase in the samples to produce different fluorescence signals, and the expression levels of firefly luciferase and renilla luciferase in the samples can be detected by chemiluminescence immunoassay analyzer.
D-xylose can produce furfural by dehydration in strong acid solution. The generated furfural reacts with Phloroglucinol to form pink compounds. The content of D-xylose can be calculated by colorimetric assay at 554 nm.
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