Ferroptosis suppressor protein-1 (FSP-1) induces non-Caspase-dependent apoptosis based on amino acid series C-terminal fragment, nuclear translocation, overexpression and other factors. The FSP-1-CoQ10-NAD(P)H pathway, parallel to the classical glutathione (GSH)-GPX4 pathway, can protect cells from ferroptosis, and play a "mixed blessing" role in cell life activities. The detection principle of this kit: FSP-1 catalyzes the substrate reaction to produce fluorescence products, and the inhibitor will inhibit the activity of FSP-1 and reduce the rate of the rise of fluorescence value. The inhibition rate can be calculated by measuring the relative change value of its enzyme activity at the excitation wavelength of 535 nm and the emission wavelength of 587 nm.
Ferrous ions (Fe2+) in samples can bind with probe to form complexes, which has a maximum absorption peak at 593 nm. The concentration of iron can be calculated by measuring the OD value at 593 nm indirectly.
Firefly Luciferin reporter gene detection is a reporting system that uses luciferin as a substrate to detect the activity of Firefly luciferase. The detection principle of this kit: In the presence of oxygen, ATP and magnesium ions at the same time, firefly luciferin is catalyzed by firefly luciferin in the sample to oxidize luciferin and emit yellow-green light, and the expression of firefly luciferin in the sample can be detected by the chemiluminescence instrument.
Firefly Luciferin reporter gene detection is a reporting system that uses luciferin as a substrate to detect the activity of Firefly luciferase. The detection principle of this kit: In the presence of oxygen, ATP and magnesium ions at the same time, firefly luciferin is catalyzed by firefly luciferase in the sample to oxidize luciferin and emit yellow-green light, and the expression of firefly luciferin in the sample can be detected by the chemiluminescence instrument. Compared with E-BC-F072, the luminescence performance of this kit is longer and more stable, and it is suitable for the detection of this system with higher expression levels and diversity.
The probe in this kit has no fluorescence outside the cell. When it enters the cell, it will emit green fluorescence after hydrolysis by the intracellular ester. The fluorescence peaks at excitation wavelength 490 nm and emission wavelength 525 nm, and the fluorescence intensity is proportional to the calcium ion concentration.
Fluoride resistant acid phosphatase (FRAP) is not inhibited by fluoride ions. It catalyzes the substrate to produce p-nitrophenol, which has a maximum absorption peak at 405 nm. The activity of FRAP can be calculated by measuring the OD value at 405 nm.
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