CM, VLDL and LDL coagulate in a polyanionic environment to form a polymer and are masked by the polymer. High-density lipoprotein (HDL) form soluble compounds under the action of a surfactant, so that HDL-C can directly react with enzyme reagents containing cholesterol esterase (CE) and cholesterol oxidase (CO) to produce hydrogen peroxide. Hydrogen peroxide is catalyzed by peroxidase (POD) in the presence of 4-aminoantipyrine (4-AA) and phenol (T-OOS) to form a red quinone compound,the shade is proportional to the amount of HDL-C.
Histamine is an endogenous active substance that exists widely in nature and is an important mediator involved in inflammatory response and immune damage. The histamine content increases with the degree of spoilage of the organism. Histamine can produce chromogenic substances with chromogenic agents under the action of enzymes, which has a maximum absorption peak at 460 nm. The histamine content of the sample can be calculated by measuring the OD value at 460 nm.
Histone deacetylases (HDAC) are a type of protease that play an important role in the structural modification of chromosomes and the regulation of gene expression. Earlier studies have shown that HDAC has a close relationship with tumors. The overexpression of HDAC can lead to various types of cancer as well as other neurological diseases, autoimmune diseases, inflammatory diseases, heart and lung diseases. Detection principle: HDAC specifically cleaves the fluorescent substrate, and the fluorescent group emits fluorescence which is detected at the excitation wavelength of 355 nm and the emission wavelength of 460 nm. Specific inhibitors are used in the kit to inhibit enzyme activity and eliminate the influence of non-specific enzyme activity.
ATPase can decompose ATP to produce ADP and inorganic phosphorus. The activity of ATPase can be expressed by measuring the production amount of inorganic phosphorus in unit time. The inorganic phosphorus reacts with ammonium molybdate in acidic solution to form ammonium molybdate compound, which is reduced with reducing agent to form molybdenum blue, and has absorption peak at 660 nm. Determine the concentration of molybdenum blue to calculate the amount of inorganic phosphorus.
Oxidative Hcy is converted into free Hcy, which can convert NADH to NAD+ through cyclic catalysis of enzymes, resulting in a decrease of OD value at 340 nm. The Hcy content in the sample is calculated by the rate of decrease.
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