Hexavalent chromium ion (Cr6+) is a highly toxic and harmful substance, which is a great threat to human health and environmental pollution. The Cr6+ pollution in the environment mainly comes from the wastewater and waste gas discharged by industries such as electroplating, leather, and metallurgy. Substances containing Cr6+ have a strong irritant effect and high permeability on the skin, which can cause damage to the cardiovascular and hematopoietic functions of the human body, and lead to diseases in organs such as the liver, kidney and lung. The detection principle of this kit is as follows: Cr6+ can react with the chromogenic agent under acidic conditions to produce a purple-red substance, with the maximum absorption at a wavelength of 540 nm. The content of Cr6+ in the water sample is calculated by measuring the OD value at 540 nm.
HK converts glucose to glucose 6-phosphate, which is catalyzed by the enzyme. Meanwhile, NAD+ is reduced to NADH, which under the action of hydrogen transmitter, transfer electrons to WST-8 to produce the yellow product. The activity of HK can be calculated by measuring the change of absorbance value at 450 nm.
High-density lipoprotein cholesterol (HDL-C), mainly synthesized in the liver, is an anti-atherosclerotic lipoprotein composed of apolipoprotein, phospholipid, cholesterol and fatty acids. The role of HDL-C is to transport phospholipids and cholesterol, which can transport cholesterol from extrahepatic tissues to the liver for metabolism, and be excreted from the body by bile acids. Plasma level of HDL-C are inversely associated with the risk of cardiovascular disease. HDL can take cholesterol from cell membranes, catalyze it into cholesterol esters by lecithin cholesterol acyltransferase, and then transfer the cholesterol ester to very low density lipoproteins and low density lipoproteins. The cholesterol content of high-density lipoprotein is relatively fixed, about 20-30% of the total cholesterol of the human body. After a series of reactions of HDL-C, the product can form a complex with the fluorescent probe. The fluorescence value of the sample at the excitation wavelength of 535 nm and the emission wavelength of 587 nm can be measured, and the content of HDL-C in the sample can be calculated.
CM, VLDL and LDL coagulate in a polyanionic environment to form a polymer and are masked by the polymer. High-density lipoprotein (HDL) form soluble compounds under the action of a surfactant, so that HDL-C can directly react with enzyme reagents containing cholesterol esterase (CE) and cholesterol oxidase (CO) to produce hydrogen peroxide. Hydrogen peroxide is catalyzed by peroxidase (POD) in the presence of 4-aminoantipyrine (4-AA) and phenol (T-OOS) to form a red quinone compound,the shade is proportional to the amount of HDL-C.
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