ELISA/CLIA/COLORIMETRIC KITS

ELISA/CLIA/COLORIMETRIC KITS

Descrizione Azione

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat BMP-4. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat BMP-4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat BMP-4, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Rat BMP-4. You can calculate the concentration of Rat BMP-4 in the samples by comparing the RLU value of the samples to the standard curve.

Codice: E-CL-R0082_48T Confezionamento: 48T
Dettagli
Codice: FY-ER1057
Dettagli
Codice: FY-ER3092
Dettagli
Codice: FY-ER4432
Dettagli
Codice: FY-ER1070
Dettagli
Codice: FY-ER4786
Dettagli
Codice: FY-ER1941
Dettagli

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Rat BNP. During the reaction, Rat BNP in the sample or standard competes with a fixed amount of Rat BNP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rat BNP. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Rat BNP in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Codice: E-EL-R0126_48T Confezionamento: 48T
Dettagli

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Rat BNP. During the reaction, Rat BNP in the sample or standard competes with a fixed amount of Rat BNP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rat BNP. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Rat BNP in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Codice: E-EL-R0126_24T Confezionamento: 24T
Dettagli

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Rat BNP. During the reaction, Rat BNP in the sample or standard competes with a fixed amount of Rat BNP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rat BNP. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Rat BNP in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Codice: E-EL-R0126_96T Confezionamento: 96T
Dettagli
Codice: FY-ER5331
Dettagli
Codice: FY-ER1951
Dettagli