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Descrizione	Azione
Monkey Perforin ELISPOT kit Codice: CT136-B2 Confezionamento: 2-plate*	Dettagli
Monkey TNF-α ELISPOT kit Codice: CT133-B5 Confezionamento: 5-plate	Dettagli
Monkey TNF-α ELISPOT kit Codice: CT133-B2 Confezionamento: 2-plate*	Dettagli
Mouse IFN-γ ELISPOT kit Codice: CT317-B2 Confezionamento: 2-plate*	Dettagli
Mouse IFN-γ ELISPOT kit Codice: CT317-B5 Confezionamento: 5-plate	Dettagli
Mouse IFN-γ ELISPOT kit Codice: CT317-B5 Confezionamento: 5-plate	Dettagli
Mouse IFN-γ ELISPOT kit Codice: CT317-B2 Confezionamento: 2-plate*	Dettagli
This assay kit uses the Sandwich-ELISA principle. The PVDF membrane at the bottom of the micro ELISPOT plate has been re-coated with anti-mouse IFN-γ antibodies. During the experiment, cell suspension and stimulants are added first, and IFN-γ secreted by the cells during the incubation process will bind to the coated antibodies. Then wash away the cells, add biotinylated anti-mouse IFN-γ antibodies and alkaline phosphatase conjugated avidin sequentially. The biotinylated anti-mouse IFN-γ antibodies will bind specifically to the IFN-γ which has already bounded, then avidin will bind specifically to biotin, together forming the complex. After free components are washed away, add chromogenic substrate (BCIP/NBT), BCIP undergoes hydrolysis under the catalysis of alkaline phosphatase and reacts with NBT, forming insoluble spots with color dark blue to blue-purple. Professional ELISPOT plate reader software can be used to analyze the result, calculating the frequency of mouse IFN-γ secreting cells. Mouse IFN-γ (Interferon Gamma) ELISPOT Kit Codice: ESP-M0001_96T Confezionamento: 96T	Dettagli
This assay kit uses the Sandwich-ELISA principle. The PVDF membrane at the bottom of the micro ELISPOT plate has been re-coated with anti-mouse IFN-γ antibodies. During the experiment, cell suspension and stimulants are added first, and IFN-γ secreted by the cells during the incubation process will bind to the coated antibodies. Then wash away the cells, add biotinylated anti-mouse IFN-γ antibodies and alkaline phosphatase conjugated avidin sequentially. The biotinylated anti-mouse IFN-γ antibodies will bind specifically to the IFN-γ which has already bounded, then avidin will bind specifically to biotin, together forming the complex. After free components are washed away, add chromogenic substrate (BCIP/NBT), BCIP undergoes hydrolysis under the catalysis of alkaline phosphatase and reacts with NBT, forming insoluble spots with color dark blue to blue-purple. Professional ELISPOT plate reader software can be used to analyze the result, calculating the frequency of mouse IFN-γ secreting cells. Mouse IFN-γ (Interferon Gamma) ELISPOT Kit Codice: ESP-M0001_96T5 Confezionamento: 96T5	Dettagli
This assay kit uses the Sandwich-ELISA principle. The PVDF membrane at the bottom of the micro ELISPOT plate has been re-coated with anti-mouse IFN-γ antibodies. During the experiment, cell suspension and stimulants are added first, and IFN-γ secreted by the cells during the incubation process will bind to the coated antibodies. Then wash away the cells, add biotinylated anti-mouse IFN-γ antibodies and alkaline phosphatase conjugated avidin sequentially. The biotinylated anti-mouse IFN-γ antibodies will bind specifically to the IFN-γ which has already bounded, then avidin will bind specifically to biotin, together forming the complex. After free components are washed away, add chromogenic substrate (BCIP/NBT), BCIP undergoes hydrolysis under the catalysis of alkaline phosphatase and reacts with NBT, forming insoluble spots with color dark blue to blue-purple. Professional ELISPOT plate reader software can be used to analyze the result, calculating the frequency of mouse IFN-γ secreting cells. Mouse IFN-γ (Interferon Gamma) solid ELISPOT Kit Codice: ESP-M0001S_96T Confezionamento: 96T	Dettagli
This assay kit uses the Sandwich-ELISA principle. The PVDF membrane at the bottom of the micro ELISPOT plate has been re-coated with anti-mouse IFN-γ antibodies. During the experiment, cell suspension and stimulants are added first, and IFN-γ secreted by the cells during the incubation process will bind to the coated antibodies. Then wash away the cells, add biotinylated anti-mouse IFN-γ antibodies and alkaline phosphatase conjugated avidin sequentially. The biotinylated anti-mouse IFN-γ antibodies will bind specifically to the IFN-γ which has already bounded, then avidin will bind specifically to biotin, together forming the complex. After free components are washed away, add chromogenic substrate (BCIP/NBT), BCIP undergoes hydrolysis under the catalysis of alkaline phosphatase and reacts with NBT, forming insoluble spots with color dark blue to blue-purple. Professional ELISPOT plate reader software can be used to analyze the result, calculating the frequency of mouse IFN-γ secreting cells. Mouse IFN-γ (Interferon Gamma) solid ELISPOT Kit Codice: ESP-M0001S_96T5 Confezionamento: 96T5	Dettagli
Mouse IgG1 B cell ELISPOT kit Codice: CT791-B5 Confezionamento: 5-plate	Dettagli

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