Descrizione Azione
Codice: CT416-B5 Confezionamento: 5-plate
Dettagli
Codice: CT416-B2 Confezionamento: 2-plate*
Dettagli
Codice: 856.151.001PC Confezionamento: 1 x 96 Discovery (pre-coated plate)
Dettagli
Codice: 856.151.002PC Confezionamento: 2 x 96 (pre-coated plates)
Dettagli
Codice: 856.171.001PC Confezionamento: 1 x 96 Discovery (pre-coated plate)
Dettagli
Codice: 856.171.002PC Confezionamento: 2 x 96 (pre-coated plates)
Dettagli
Codice: 856.171.005PC Confezionamento: 5 x 96 (pre-coated plates)
Dettagli

This assay kit uses the Sandwich-ELISA principle. The PVDF membrane at the bottom of the micro ELISPOT plate has been re-coated with anti-human IL-17A antibodies. During the experiment, cell suspension and stimulants are added first, and IL-17A secreted by the cells during the incubation process will bind to the coated antibodies. Then wash away the cells, add biotinylated anti-human IL-17A antibodies and alkaline phosphatase conjugated avidin sequentially. The biotinylated anti-human IL-17A antibodies will bind specifically to the IL-17A which has already bounded, then avidin will bind specifically to biotin, together forming the complex. After free components are washed away, add chromogenic substrate (BCIP/NBT), BCIP undergoes hydrolysis under the catalysis of alkaline phosphatase and reacts with NBT, forming insoluble spots with color dark blue to blue-purple. Professional ELISPOT plate reader software can be used to analyze the result, calculating the frequency of human IL-17A secreting cells.

Codice: ESP-H0004_96T*5 Confezionamento: 96T*5
Dettagli

This assay kit uses the Sandwich-ELISA principle. The PVDF membrane at the bottom of the micro ELISPOT plate has been re-coated with anti-human IL-17A antibodies. During the experiment, cell suspension and stimulants are added first, and IL-17A secreted by the cells during the incubation process will bind to the coated antibodies. Then wash away the cells, add biotinylated anti-human IL-17A antibodies and alkaline phosphatase conjugated avidin sequentially. The biotinylated anti-human IL-17A antibodies will bind specifically to the IL-17A which has already bounded, then avidin will bind specifically to biotin, together forming the complex. After free components are washed away, add chromogenic substrate (BCIP/NBT), BCIP undergoes hydrolysis under the catalysis of alkaline phosphatase and reacts with NBT, forming insoluble spots with color dark blue to blue-purple. Professional ELISPOT plate reader software can be used to analyze the result, calculating the frequency of human IL-17A secreting cells.

Codice: ESP-H0004_96T Confezionamento: 96T
Dettagli

This assay kit uses the Sandwich-ELISA principle. The PVDF membrane at the bottom of the micro ELISPOT plate has been re-coated with anti-human IL-17A antibodies. During the experiment, cell suspension and stimulants are added first, and IL-17A secreted by the cells during the incubation process will bind to the coated antibodies. Then wash away the cells, add biotinylated anti-human IL-17A antibodies and alkaline phosphatase conjugated avidin sequentially. The biotinylated anti-human IL-17A antibodies will bind specifically to the IL-17A which has already bounded, then avidin will bind specifically to biotin, together forming the complex. After free components are washed away, add chromogenic substrate (BCIP/NBT), BCIP undergoes hydrolysis under the catalysis of alkaline phosphatase and reacts with NBT, forming insoluble spots with color dark blue to blue-purple. Professional ELISPOT plate reader software can be used to analyze the result, calculating the frequency of human IL-17A secreting cells.

Codice: ESP-H0004S_96T Confezionamento: 96T
Dettagli

This assay kit uses the Sandwich-ELISA principle. The PVDF membrane at the bottom of the micro ELISPOT plate has been re-coated with anti-human IL-17A antibodies. During the experiment, cell suspension and stimulants are added first, and IL-17A secreted by the cells during the incubation process will bind to the coated antibodies. Then wash away the cells, add biotinylated anti-human IL-17A antibodies and alkaline phosphatase conjugated avidin sequentially. The biotinylated anti-human IL-17A antibodies will bind specifically to the IL-17A which has already bounded, then avidin will bind specifically to biotin, together forming the complex. After free components are washed away, add chromogenic substrate (BCIP/NBT), BCIP undergoes hydrolysis under the catalysis of alkaline phosphatase and reacts with NBT, forming insoluble spots with color dark blue to blue-purple. Professional ELISPOT plate reader software can be used to analyze the result, calculating the frequency of human IL-17A secreting cells.

Codice: ESP-H0004S_96T*5 Confezionamento: 96T*5
Dettagli
Codice: CT418-B5 Confezionamento: 5-plate
Dettagli