VCP(Valosin-containing protein) belongs to the AAA ATPase family.VCP was first identified as a result of attempts to clone a putative peptide hormone called valosin. It was found that the cloned cDNA encoded a ubiquitously expressed 90 kDa cytosolic protein,termed VCP,which showed none of the characteristics of a peptide hormone precursor.Defects in VCP are the cause of inclusion body myopathy with early-onset Paget disease and frontotemporal dementia (IBMPFD) and amyotrophic lateral sclerosis type 14 with or without frontotemporal dementia (ALS14)
VCY; VCY1B Antibody detects endogenous levels of VCY; VCY1B.
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal VD3. During the reaction, Universal VD3 in the sample or standard competes with a fixed amount of Universal VD3 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal VD3. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal VD3 in tested samples can be calculated by comparing the OD of the samples to the standard curve.
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