Hydrogen peroxide can react with ammonium molybdate to form a yellow complex which has a maximum absorption peak at 405 nm. H2O2 content can be calculated by measuring the absorbance value at 405 nm.
In the presence of peroxidase, hydrogen peroxide reacts with the fluorescent probe, and the fluorescence intensity at the excitation wavelength of 535 nm and the emission wavelength of 587 nm is proportional to the hydrogen peroxide concentration.
H2S can be absorbed by acetate solution to form ZnS, which can form methylene blue in the presence of Fe3+ and chromogenic agent. Methylene blue has a maximum absorption peak at 680 nm. H2S content can be calculated indirectly by measuring the OD value at 680 nm.
Hydroxyl radical is a kind of reactive oxygen species, which can kill red blood cells, degrade DNA, cell membrane and polysaccharide compounds, causing damage to cell structure and function, and then leading to metabolic disorders in the body to cause diseases. The scavenging ability of hydroxyl free radical is one of the important indexes of the antioxidant ability of samples. It has been widely used in the research of antioxidant health care products and drugs.
he Fenton reaction is the most common chemical reaction that generates hydroxyl radicals (OH·). The amount of hydrogen peroxide is directly proportional to the amount of OH· produced by the Fenton reaction. After the electron accepter is given, adding griess reagent to produce the red substance, which is directly proportional to the amount of OH·. The substances contained in the sample that can eliminate hydroxyl free radical can consume OH·. The remaining OH· is detected to reflect hydroxyl free radical scavenging capacity.
The sample is hydrolyzed to generate free HYP, and hydroxyproline can produce oxidation product under the action of oxidizing agent. The generated oxidation product can react with chromogenic agent to produce burgundy. The concentration of hydroxyproline can be calculated by measuring the OD value at 558 nm.
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