Formic acid is the simplest carboxylic acid, whose chemical formula is CH2O2. It is often used as an antimicrobial/preservative in livestock feed. Formic acid can block some of the decay processes in feed, making the nutritional value of feed maintain longer. Under normal circumstances, the physiological concentration of formic acid is low and easy to metabolize, but in the case of methanol poisoning, the concentration can reach 5 mmoL/L. Similarly, long-term exposure to excessive levels of formaldehyde can also increase the content of formic acid in blood and urine.
Free cholesterol produces 4-cholestenone and hydrogen peroxide under the oxidation of cholesterol oxidase. In the presence of 4-aminoamylpyridine and phenol, peroxidase catalyze hydrogen peroxide to form red quinone compounds of benzoquinone imine phenizone. The color depth of the generated quinones is directly proportional to the cholesterol content.
Free fatty acids (NEFA/FFA) generate acyl-CoA under the action of acyl synthase, then acyl-CoA generates hydrogen peroxide under the oxidation of acyl oxidase. Hydrogen peroxide reacts in the presence of enzymes and chromogens, producing a red substance with maximum absorption at 546 nm. The amount of this red product is proportional to the content of free fatty acids.
Free fatty acids produce acyl coenzyme A in the presence of acyl synthase, which produces hydrogen Free fatty acids produce acyl coenzyme A in the presence of acyl synthase, which produces hydrogen peroxide in the presence of acyl oxidase. In the presence of the enzyme and probe, hydrogen peroxide react to produce the fluorescence substrate. The fluorescence intensity at the excitation wavelength of 535 nm and emission wavelength of 590 nm is directly proportional to the concentration of free fatty acids.
Fructose-6-phosphate (F-6-P) is a six-carbon sugar formed by esterification of fructose and phosphate. It is an important intermediate in glycolysis and gluconeogenesis pathways. As the most basic metabolic substances in the metabolic process of living bodies and cells, it is of great significance to study them quantitatively. Enzyme catalyze F-6-P to produce a specific product, which can react with chromogenic agent to produce the fluorescent substance. Detect the fluorescent intensity of fluorescent substance at the excitation wavelength of 535 nm and the emission wavelength of 587 nm.
Fructose is one of the most common hexaketose sugars, which is abundant as a monosaccharide in free form in fruits and honey. A large number of epidemiological data and experimental studies have shown that excessive fructose intake may be an important factor in the increased incidence of metabolic diseases. Fructose can interact with the matrix solution to form a specific product, which has a maximum absorption peak at 285 nm.
Fructose is the most common hexketoose, which is often present in large amounts as a free monosaccharide in fruits and honey. A large number of epidemiological data and experimental studies have shown that excessive fructose intake may be an important factor in the increased incidence of metabolic diseases. Fructose can produce a specific product under the action of enzymes, which reacts with the chromogenic agent to produce a fluorescent substance (Ex/Em: 530 nm/590 nm).
Fumarase (FUM), also known as malate lyase, fumarate hydrase or fumarase, is an important enzyme in the tricarboxylic acid cycle, which can catalyze the hydration of fumarase to synthesize malic acid. FUM is a kind of intracellular enzyme that exists widely in plants and animals. The detection principle of this kit: fumarase catalyze fumaric acid products under the action of electronic coupler to reduce the chromogenic reagent to orange products, detection at about 450 nm has the maximum absorption peak.
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