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Descrizione	Azione
Human IL-4 ELISPOT kit Codice: CT232-B2 Confezionamento: 2-plate*	Dettagli
Human IL-4 ELISpot Kit - 1x96 tests (pre-coated plates) Codice: 856.011.001PC Confezionamento: 1 x 96 Discovery (pre-coated plate)	Dettagli
Human IL-4 ELISpot Kit - 2x96 tests (pre-coated plates) Codice: 856.011.002PC Confezionamento: 2 x 96 (pre-coated plates)	Dettagli
Human IL-4 ELISpot Kit - 5x96 tests (pre-coated plates) Codice: 856.011.005PC Confezionamento: 5 x 96 (pre-coated plates)	Dettagli
This assay kit uses the Sandwich-ELISA principle. The PVDF membrane at the bottom of the micro ELISPOT plate has been re-coated with anti-human IL-4 antibodies. During the experiment, cell suspension and stimulants are added first, and IL-4 secreted by the cells during the incubation process will bind to the coated antibodies. Then wash away the cells, add biotinylated anti-human IL-4 antibodies and alkaline phosphatase conjugated avidin sequentially. The biotinylated anti-human IL-4 antibodies will bind specifically to the IL-4 which has already bounded, then avidin will bind specifically to biotin, together forming the complex. After free components are washed away, add chromogenic substrate (BCIP/NBT), BCIP undergoes hydrolysis under the catalysis of alkaline phosphatase and reacts with NBT, forming insoluble spots with color dark blue to blue-purple. Professional ELISPOT plate reader software can be used to analyze the result, calculating the frequency of human IL-4 secreting cells. Human IL-4 (Interleukin 4) ELISPOT Kit Codice: ESP-H0007_96T5 Confezionamento: 96T5	Dettagli
This assay kit uses the Sandwich-ELISA principle. The PVDF membrane at the bottom of the micro ELISPOT plate has been re-coated with anti-human IL-4 antibodies. During the experiment, cell suspension and stimulants are added first, and IL-4 secreted by the cells during the incubation process will bind to the coated antibodies. Then wash away the cells, add biotinylated anti-human IL-4 antibodies and alkaline phosphatase conjugated avidin sequentially. The biotinylated anti-human IL-4 antibodies will bind specifically to the IL-4 which has already bounded, then avidin will bind specifically to biotin, together forming the complex. After free components are washed away, add chromogenic substrate (BCIP/NBT), BCIP undergoes hydrolysis under the catalysis of alkaline phosphatase and reacts with NBT, forming insoluble spots with color dark blue to blue-purple. Professional ELISPOT plate reader software can be used to analyze the result, calculating the frequency of human IL-4 secreting cells. Human IL-4 (Interleukin 4) ELISPOT Kit Codice: ESP-H0007_96T Confezionamento: 96T	Dettagli
This assay kit uses the Sandwich-ELISA principle. The PVDF membrane at the bottom of the micro ELISPOT plate has been re-coated with anti-human IL-4 antibodies. During the experiment, cell suspension and stimulants are added first, and IL-4 secreted by the cells during the incubation process will bind to the coated antibodies. Then wash away the cells, add biotinylated anti-human IL-4 antibodies and alkaline phosphatase conjugated avidin sequentially. The biotinylated anti-human IL-4 antibodies will bind specifically to the IL-4 which has already bounded, then avidin will bind specifically to biotin, together forming the complex. After free components are washed away, add chromogenic substrate (BCIP/NBT), BCIP undergoes hydrolysis under the catalysis of alkaline phosphatase and reacts with NBT, forming insoluble spots with color dark blue to blue-purple. Professional ELISPOT plate reader software can be used to analyze the result, calculating the frequency of human IL-4 secreting cells. Human IL-4 (Interleukin 4) solid ELISPOT Kit Codice: ESP-H0007S_96T5 Confezionamento: 96T5	Dettagli
This assay kit uses the Sandwich-ELISA principle. The PVDF membrane at the bottom of the micro ELISPOT plate has been re-coated with anti-human IL-4 antibodies. During the experiment, cell suspension and stimulants are added first, and IL-4 secreted by the cells during the incubation process will bind to the coated antibodies. Then wash away the cells, add biotinylated anti-human IL-4 antibodies and alkaline phosphatase conjugated avidin sequentially. The biotinylated anti-human IL-4 antibodies will bind specifically to the IL-4 which has already bounded, then avidin will bind specifically to biotin, together forming the complex. After free components are washed away, add chromogenic substrate (BCIP/NBT), BCIP undergoes hydrolysis under the catalysis of alkaline phosphatase and reacts with NBT, forming insoluble spots with color dark blue to blue-purple. Professional ELISPOT plate reader software can be used to analyze the result, calculating the frequency of human IL-4 secreting cells. Human IL-4 (Interleukin 4) solid ELISPOT Kit Codice: ESP-H0007S_96T Confezionamento: 96T	Dettagli
Human IL-5 ELISPOT kit Codice: CT233-B2 Confezionamento: 2-plate*	Dettagli
Human IL-5 ELISPOT kit Codice: CT233-B5 Confezionamento: 5-plate	Dettagli
Human IL-5 ELISPOT kit Codice: CT233-B5 Confezionamento: 5-plate	Dettagli
Human IL-5 ELISPOT kit Codice: CT233-B2 Confezionamento: 2-plate*	Dettagli

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