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Descrizione	Azione
Human Perforin ELISpot Kit - 5x96 tests (pre-coated plates) Codice: 856.141.001PC Confezionamento: 1 x 96 Discovery (pre-coated plate)	Dettagli
Human Perforin ELISpot Kit - 5x96 tests (pre-coated plates) Codice: 856.141.005PC Confezionamento: 5 x 96 (pre-coated plates)	Dettagli
Human TNF-a ELISpot Kit - 2x96 tests (pre-coated plates) Codice: 856.041.002PC Confezionamento: 2 x 96 (pre-coated plates)	Dettagli
Human TNF-a ELISpot Kit - 5x96 tests (pre-coated plates) Codice: 856.041.005PC Confezionamento: 5 x 96 (pre-coated plates)	Dettagli
Human TNF-a pre-coated ELISpot Kit - 1x96 tests Codice: 856.041.001PC Confezionamento: 1 x 96 Discovery (pre-coated plate)	Dettagli
Human TNF-α ELISPOT kit Codice: CT237-B5 Confezionamento: 5-plate	Dettagli
Human TNF-α ELISPOT kit Codice: CT237-B2 Confezionamento: 2-plate*	Dettagli
Human TNF-α ELISPOT kit Codice: CT237-B2 Confezionamento: 2-plate*	Dettagli
Human TNF-α ELISPOT kit Codice: CT237-B5 Confezionamento: 5-plate	Dettagli
This assay kit uses the Sandwich-ELISA principle. The PVDF membrane at the bottom of the micro ELISPOT plate has been re-coated with anti-human TNF-α antibodies. During the experiment, cell suspension and stimulants are added first, and TNF-α secreted by the cells during the incubation process will bind to the coated antibodies. Then wash away the cells, add biotinylated anti-human TNF-α antibodies and alkaline phosphatase conjugated avidin sequentially. The biotinylated anti-human TNF-α antibodies will bind specifically to the TNF-α which has already bounded, then avidin will bind specifically to biotin, together forming the complex. After free components are washed away, add chromogenic substrate (BCIP/NBT), BCIP undergoes hydrolysis under the catalysis of alkaline phosphatase and reacts with NBT, forming insoluble spots with color dark blue to blue-purple. Professional ELISPOT plate reader software can be used to analyze the result, calculating the frequency of human TNF-α secreting cells. Human TNF-α (Tumor Necrosis Factor Alpha) ELISPOT Kit Codice: ESP-H0010_96T Confezionamento: 96T	Dettagli
This assay kit uses the Sandwich-ELISA principle. The PVDF membrane at the bottom of the micro ELISPOT plate has been re-coated with anti-human TNF-α antibodies. During the experiment, cell suspension and stimulants are added first, and TNF-α secreted by the cells during the incubation process will bind to the coated antibodies. Then wash away the cells, add biotinylated anti-human TNF-α antibodies and alkaline phosphatase conjugated avidin sequentially. The biotinylated anti-human TNF-α antibodies will bind specifically to the TNF-α which has already bounded, then avidin will bind specifically to biotin, together forming the complex. After free components are washed away, add chromogenic substrate (BCIP/NBT), BCIP undergoes hydrolysis under the catalysis of alkaline phosphatase and reacts with NBT, forming insoluble spots with color dark blue to blue-purple. Professional ELISPOT plate reader software can be used to analyze the result, calculating the frequency of human TNF-α secreting cells. Human TNF-α (Tumor Necrosis Factor Alpha) ELISPOT Kit Codice: ESP-H0010_96T5 Confezionamento: 96T5	Dettagli
This assay kit uses the Sandwich-ELISA principle. The PVDF membrane at the bottom of the micro ELISPOT plate has been re-coated with anti-human TNF-α antibodies. During the experiment, cell suspension and stimulants are added first, and TNF-α secreted by the cells during the incubation process will bind to the coated antibodies. Then wash away the cells, add biotinylated anti-human TNF-α antibodies and alkaline phosphatase conjugated avidin sequentially. The biotinylated anti-human TNF-α antibodies will bind specifically to the TNF-α which has already bounded, then avidin will bind specifically to biotin, together forming the complex. After free components are washed away, add chromogenic substrate (BCIP/NBT), BCIP undergoes hydrolysis under the catalysis of alkaline phosphatase and reacts with NBT, forming insoluble spots with color dark blue to blue-purple. Professional ELISPOT plate reader software can be used to analyze the result, calculating the frequency of human TNF-α secreting cells. Human TNF-α (Tumor Necrosis Factor Alpha) solid ELISPOT Kit Codice: ESP-H0010S_96T5 Confezionamento: 96T5	Dettagli

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