Descrizione Azione
Codice: 856.021.002PC Confezionamento: 2 x 96 (pre-coated plates)
Dettagli
Codice: 856.021.005PC Confezionamento: 5 x 96 (pre-coated plates)
Dettagli

This assay kit uses the Sandwich-ELISA principle. The PVDF membrane at the bottom of the micro ELISPOT plate has been re-coated with anti-human IL-6 antibodies. During the experiment, cell suspension and stimulants are added first, and IL-6 secreted by the cells during the incubation process will bind to the coated antibodies. Then wash away the cells, add biotinylated anti-human IL-6 antibodies and alkaline phosphatase conjugated avidin sequentially. The biotinylated anti-human IL-6 antibodies will bind specifically to the IL-6 which has already bounded, then avidin will bind specifically to biotin, together forming the complex. After free components are washed away, add chromogenic substrate (BCIP/NBT), BCIP undergoes hydrolysis under the catalysis of alkaline phosphatase and reacts with NBT, forming insoluble spots with color dark blue to blue-purple. Professional ELISPOT plate reader software can be used to analyze the result, calculating the frequency of human IL-6 secreting cells.

Codice: ESP-H0009_96T*5 Confezionamento: 96T*5
Dettagli

This assay kit uses the Sandwich-ELISA principle. The PVDF membrane at the bottom of the micro ELISPOT plate has been re-coated with anti-human IL-6 antibodies. During the experiment, cell suspension and stimulants are added first, and IL-6 secreted by the cells during the incubation process will bind to the coated antibodies. Then wash away the cells, add biotinylated anti-human IL-6 antibodies and alkaline phosphatase conjugated avidin sequentially. The biotinylated anti-human IL-6 antibodies will bind specifically to the IL-6 which has already bounded, then avidin will bind specifically to biotin, together forming the complex. After free components are washed away, add chromogenic substrate (BCIP/NBT), BCIP undergoes hydrolysis under the catalysis of alkaline phosphatase and reacts with NBT, forming insoluble spots with color dark blue to blue-purple. Professional ELISPOT plate reader software can be used to analyze the result, calculating the frequency of human IL-6 secreting cells.

Codice: ESP-H0009_96T Confezionamento: 96T
Dettagli

This assay kit uses the Sandwich-ELISA principle. The PVDF membrane at the bottom of the micro ELISPOT plate has been re-coated with anti-human IL-6 antibodies. During the experiment, cell suspension and stimulants are added first, and IL-6 secreted by the cells during the incubation process will bind to the coated antibodies. Then wash away the cells, add biotinylated anti-human IL-6 antibodies and alkaline phosphatase conjugated avidin sequentially. The biotinylated anti-human IL-6 antibodies will bind specifically to the IL-6 which has already bounded, then avidin will bind specifically to biotin, together forming the complex. After free components are washed away, add chromogenic substrate (BCIP/NBT), BCIP undergoes hydrolysis under the catalysis of alkaline phosphatase and reacts with NBT, forming insoluble spots with color dark blue to blue-purple. Professional ELISPOT plate reader software can be used to analyze the result, calculating the frequency of human IL-6 secreting cells.

Codice: ESP-H0009S_96T*5 Confezionamento: 96T*5
Dettagli

This assay kit uses the Sandwich-ELISA principle. The PVDF membrane at the bottom of the micro ELISPOT plate has been re-coated with anti-human IL-6 antibodies. During the experiment, cell suspension and stimulants are added first, and IL-6 secreted by the cells during the incubation process will bind to the coated antibodies. Then wash away the cells, add biotinylated anti-human IL-6 antibodies and alkaline phosphatase conjugated avidin sequentially. The biotinylated anti-human IL-6 antibodies will bind specifically to the IL-6 which has already bounded, then avidin will bind specifically to biotin, together forming the complex. After free components are washed away, add chromogenic substrate (BCIP/NBT), BCIP undergoes hydrolysis under the catalysis of alkaline phosphatase and reacts with NBT, forming insoluble spots with color dark blue to blue-purple. Professional ELISPOT plate reader software can be used to analyze the result, calculating the frequency of human IL-6 secreting cells.

Codice: ESP-H0009S_96T Confezionamento: 96T
Dettagli
Codice: CT681-B2 Confezionamento: 2-plate*
Dettagli
Codice: CT681-B5 Confezionamento: 5-plate
Dettagli
Codice: CT681-B5 Confezionamento: 5-plate
Dettagli
Codice: CT681-B2 Confezionamento: 2-plate*
Dettagli
Codice: 856.141.002PC Confezionamento: 2 x 96 (pre-coated plates)
Dettagli
Codice: 856.131.005PC Confezionamento: 5 x 96 (pre-coated plates)
Dettagli